Dr. Leopold L. Ilag's research interests are on proteomics, membrane protein biochemistry, structural biology, and bioanalytical chemistry. His specialties finds application on research with interests on the development and application of new mass spectrometric methods to address problems in the life sciences. Focus is mainly on molecular machines and membrane proteins. Previous work in Carol Robinson’s group has been on the characterization of macromolecular complexes such as RNA polymerase, degradosome and the ribosome as well as membrane proteins like EmrE, a bacterial drug resistance pump. The Hoya ilagii flower is a wild species named collectively for their Ilag family. It is found in the Island of Luzon, Philippines.
Sex: Male
Education:
- The Karolinska Institute Stockholm, Sweden, PH.D. Molecular Neurobiology / Medical Biochemistry and Biophysics, 1997
- University of the Philippines, Los Baños, Los Baños, Laguna, BS Biology ( Magna Cum Laude), 1991
Field of Specialization:
Bioanalytical Chemistry Biological & Environmental Proteomics, Biological & Environmental Proteomics, Biochemistry, Physics, Mass Spectrometry of Molecular Machines and Membrane proteins
Researches:
Tamburro, Davide, Ilag, L., et al., (2011) Multifunctional Core-Shell Nanoparticles: Discovery of Previously Invisible Biomarkers
Journal of American Chemical Society Volume 133, No. 47
M. Shariatgorji; Astorga-Wells, Juan; L. Ilag, (2011) Trends in the bioanalytical applications of microfluidic electrocapture
Analytical and Bioanalytical Chemistry Volume 399
Maddalo, G., Ilag, L., et al., (2011) Systematic Analysis of Native Membrane Protein Complexes in Escherichia coli
Journal of Proteome Research Volume 10, No. 4
Maddalo, G., Peter C., Stenberg-Bruzell, F., Nielsen, H.V., Jensen-Seaman, M., Ilag, L., Kline, Kimberly, K., Daniel, D., (2011) A reference map of the membrane proteome of Enterococcus faecalis
Proteomics Volume 11
Kärkäs, Markus, Shariatgorji, M., Ilag, L., et al, (2011) Light-Induced Water Oxidation by a Ru-complex Containing a Bio-Inspired Ligand
Chemistry Volume 17
Gao, Qiuju, Nilsson, U., Ilag, L., Leck, Caroline, (2011) Monosaccharide compositional analysis of marine polysaccharides by hydrophilic interaction liquid chromatography-tandem mass spectrometry
Analytical and Bioanalytical Chemistry Volume 399, No. 7
Douglas, T.A., Tamburro, Davide, Ilag, L.,et al., (2011) The use of hydrogel microparticles to sequester and concentrate bacterial antigens in a urine test for Lyme disease
Biomaterials Volume 4
Aminlashgari, Nina; M. Shariatgorji; L. Ilag; Hakkarainen, Minna, (2011) Nanocomposites as novel surfaces for laser desorption ionization mass spectrometry
Analytical Methods Volume 3
Z. Spacil; Eriksson J; Jonasson S; Rasmussen U; L. Ilag; Bergman B, (2010) Analytical protocol for identification of BMAA and DAB in biologicalsamples
Analyst Volume 135
Maddalo, G., Shariatgorji, M., Adams, C., Fung, E., Nilsson, U., Zubarev, R., Ilag, L., (2010) Porcine P2 myelin protein primary structure and bound fatty acids determined by mass spectrometry
Analytical and Bioanalytical Chemistry Volume 397, No. 5
Jonasson, S., Eriksson, J., Berntzon, L., Spacil, Z., Ilag, L., Ronnevi, L, et al, (2010) Transfer of a cyanobacterial neurotoxin within a temperate aquatic ecosystem suggests pathways for human exposure
Proc Natl Acad Sci U S A Volume 107, No. 20
Redeby, T., Nilsson, U., Ilag, L., et al, (2010) Specific Adducts Formed through a Radical Reaction between Peptides and Contact Allergenic Hydroperoxides
Chemical Research in Toxicology Volume 23, No. 1
Leonova, E., Grins, J., Shariatgorji, M., Ilag, L.L., Edén, M., (2009) Solid-state NMR investigations of Si-29 and N-15 enriched silicon nitride
Solid State Nuclear Magnetic Resonance Volume 1
Shariatgorji, M., Amini, N., Ilag, L., (2009) Silicon nitride nanoparticles for surface-assisted laser desorption/ionization of small molecules
Journal of Nanoparticle Research Volume 11, No. 6
Spacil, Z., Shariatgorji, M., Amini, N., Solich, P., Ilag, L., (2009) Matrix-less laser desorption/ionisation mass spectrometry of polyphenols in red wine
Rapid Communication Mass Spectrometry Volume 23, No. 12
Shariatgorji, M., Astorga-Wells, J., Jornvall, H., Ilag, L., (2008) Microfluidic Electrocapture-Assisted Mass Spectrometry of Membrane-Associated Polypeptides Abstract
Analytical Chemistry Volume 18
Shariatgorji, M., Amini, N., Thorsén, G., Crescenzi, C., Ilag, L., (2008) ì-Trap for the SALDI-MS Screening of Organic Compounds Prior to LC/MS Analysis
Analytical Chemistry Volume 80, No. 14
Pisareva, T., Shumskaya, M., Maddalo, G., Ilag, L., Norling, B., (2007) Proteomics of Synechocystis sp. PCC 6803. Identification of novel integral plasma membrane proteins
FEBS Letters Volume 274
Sharon, M., Ilag, L., Robinson, C.V., (2007) Evidence for Micellar Structure in the Gas Phase
Journal of American Chemical Society Volume 129
Rundlof, A.K., Fernandes, A.P.; Selenius, M.; Babic, M.; M. Shariatgorji; Nilsonne, G; L. Ilag; Dobra, K.; Bjornstedt, M., (2007) Quantification of alternative mRNA species and identification of thioredoxin reductase 1 isoforms in human tumor cells
Differentiation Volume 75
McKay, A.R., Ruotolo, B.T., L. Ilag, L., Robinson, C.V., (2006) Mass Measurement of Increased Accuracy Resolve Heterogeneous Populations of Intact Ribosomes
Journal of American Chemical Society Volume 128, No. 35
Bader, R., Seeliger, M.A., Kelly, S.E., Ilag, L.L., Meersman, F., Limones, A., Luisi, B.F., Dobson, C.M., Itzhaki, L.S., (2006) Folding and fibril formation of the cell cycle protein Cks1
Journal of Biological Chemistry Volume 281, No. 27
Harmer, N.J., Robinson, C.J., Adam, L.E., Ilag, L., Robinson, C.V., Gallagher, J.T., Blundell, T.L., (2005) Multimers of the fibroblast growth factor (FGF)-FGF receptor-saccharide complex are formed on long oligomers of heparin
Biochemistry Journal
Stenberg F; Chovanec P; Maslen SL; Robinson CV; L. Ilag; von Heijne G; Daley DO, (2005) Protein Complexes of the Escherichia coli Cell Envelope
Journal of Biological Chemistry Volume 280, No. 41
Ilag, L., Videler, H., McKay, A.R., Sobott, F., Fucini, P., Nierhaus, K.H., Robinson, C.V., (2005) Heptameric (L12)6/L10 rather than canonical pentameric complexes are found by tandem MS of intact ribosomes from thermophilic bacteria
Proceedings of the National Academy of Science and Techology, U.S.A. Volume 23
Videler, H., Ilag, L., MacKay, A.R.C., Hanson, C.L., Robinson, C.V., (2005) Mass Spectrometry of intact ribosomes
FEBS Letters Volume 579, No. 4
Ilag, L., Ubarettxenia-Belandia, I., Tate, C.G., Robinson, C.V., (2004) Drug Binding Revealed by Tandem Mass Spectrometry of a Protein-Micelle Complex
Journal of American Chemical Society Volume 126, No. 44
Westblade, L.F., Ilag, L., Powell, K., Scott, D.V., Kolb, A., Robinson, C.V., Busby, S.J.W., (2004) Studies of the Escherichia coli Rsd-sigma 70 complex
Journal of Molecular Biology Volume 335
Ilag, L.L., Westblade, L.F., Kolb, A., Busby, S.W.J., Robinson, C.V., (2004) Mass Spectrometry of Escherichia coli RNA Polymerase; Interactions of the Core Enzyme with sigma(70) and Rsd Protein
Structure Volume 12, No. 2
Harmer, N., Ilag, L., Mulloy, B., Pellegrini, L., Robinson, C.V., Blundell, T.L., (2004) Towards a resolution of the stoichiometry of the Fibroblast Growth Factor (FGF)-FGF Receptor-heparin complex
Journal of Molecular Bology Volume 339, No. 4
Maddalo, G., Shariatgorji, M., Adams, C., Fung, E., Nilsson, U., Zubarev, R., Ilag, L., (2010) Porcine P2 myelin protein primary structure and bound fatty acids determined by mass spectrometry
Analytical and Bioanalytical Chemistry Volume 397, No. 5
Papers Presented:
- Microfluidic electrocapture-assisted mass spectrometry of membrane-associated polypeptides, Department of Analytical Chemistry, Stockholm University, 10691 Stockholm, Sweden
- Porcine P2 myelin protein primary structure and bound fatty acids determined by mass spectrometry, Department of Analytical Chemistry, Stockholm University, Svante Arrhenius väg 16, 10691,Stockholm, Sweden
- Strategy for quantifying trace levels of BMAA in cyanobacteria by LC/MS/MS, Department of Analytical Chemistry, Stockholm University, 10691, Stockholm, Sweden
Award:
- Balik Scientist Award, DOST, 2008